I could not understand why HPLC test has been advised. If your wife is chronically anaemic then this test can be done.
Rubella IgG test is also of doubtful value if done when the lady is trying to conceive.
IgM antibodies are dangerous because they signifying existing infection which can be transmitted to the baby.
IgG signifies old infection which may be burnt out. So it can't be transmitted to the baby and this test will be positive for a very long time.
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Disclaimer : The content is not intended to be a substitute for professional medical advice, diagnosis, or treatment. Always seek the advice of your physician or other qualified health provider with any questions you may have regarding your medical condition. Never disregard professional medical advice or delay in seeking it because of something you have read on this website.
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